![]() The MOp lacks species-specific cellular structures, such as the whisker barrels in the rodent primary somatosensory cortex and the elaborate layer 4 (L4) with multiple sublayers in the primate primary visual cortex. 1a–d) as the starting point for our joint efforts owing to its relatively conserved structure and function across mammalian species. We selected the primary motor cortex (MOp) (Extended Data Fig. Within the BRAIN Initiative Cell Census Network (BICCN), we aim to create an atlas of cell types across the brain of several mammalian species by integrating multiple single-cell omics approaches. Molecular and genomic cell signatures will drive progress across modalities and help to obtain functional information. Although a comprehensive cell atlas should incorporate anatomical and physiological information, the high throughput of single-cell sequencing assays presents an opportunity for establishing a broad-based transcriptomic and epigenomic cell atlas. As the number of profiled cells grows into the millions, a key question is whether these data will converge towards a comprehensive, coherent taxonomy. ![]() Recent advances include single-cell transcriptomic datasets with more than 10 5 individual cells, identifying hundreds of neuronal and non-neuronal cell types across the mouse nervous system 1, 2, 3. Single-cell molecular assays, especially transcriptomic measurements by RNA sequencing (RNA-seq), have accelerated the discovery of cell types across brain regions and in diverse species 7. The cellular components of brain circuits are extraordinarily diverse 5, 6. Our results highlight the complex molecular regulation of cell types in the brain and will directly enable the design of reagents to target specific cell types in the mouse primary motor cortex for functional analysis. We further discovered thousands of concordant marker genes and gene regulatory elements for these cell types. The atlas includes a population of excitatory neurons that resemble pyramidal cells in layer 4 in other cortical regions 4. The resulting reference atlas-containing over 56 neuronal cell types that are highly replicable across analysis methods, sequencing technologies and modalities-is a comprehensive molecular and genomic account of the diverse neuronal and non-neuronal cell types in the mouse primary motor cortex. ![]() We developed computational and statistical methods to integrate multimodal data and quantitatively validate cell-type reproducibility. Here we generated transcriptomes and epigenomes from more than 500,000 individual cells in the mouse primary motor cortex, a structure that has an evolutionarily conserved role in locomotion. With the proliferation of multi-omics datasets, a major challenge is to validate and integrate results into a biological understanding of cell-type organization. Single-cell transcriptomics can provide quantitative molecular signatures for large, unbiased samples of the diverse cell types in the brain 1, 2, 3. ![]() Nature volume 598, pages 103–110 ( 2021) Cite this article A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |